Subject(s)
Humans , Animals , Cells, Cultured/virology , Hepacivirus/genetics , Hepacivirus/physiology , Hepatocytes/virology , Virus Replication , Cell Line , DNA, Viral/analysis , Genes, Viral , Pan troglodytes , Replicon , Reverse Transcriptase Polymerase Chain Reaction , RNA, Viral/analysis , Virus Replication/genetics , Virus CultivationABSTRACT
To establish if BTV was circulating in Argentina, 94 bovines from the Santo TomÚ and Ituzaingó Departments of Corrientes Province were sampled every 30-60 days during 14 months. Red blood cells from those animals that showed seroconvertion with a c-ELISA were processed for virus isolation by inoculation in embryonated chicken eggs and cell cultures. Cells with CPE were positive by direct and indirect immunofluorescence with BTV specific reagents. These samples examined by electron microscopy showed virus particles with BTV morphological characteristics. Blood samples and tissue culture supernantants were positive by RT-PCR technique with primers corresponding to the segment 3 of the BTV genome. Haematophagous insects were captured in one farm using light traps and Culicoides insignis Lutz was the predominant species detected. This is the first isolation of BTV in Argentina from northeastern bovines without any disease symptom.
Subject(s)
Animals , Cattle , Bluetongue , Ceratopogonidae , Cattle Diseases/virology , Insect Vectors , Bluetongue virus/isolation & purification , Antibodies, Viral , Argentina , Bluetongue , Cells, Cultured/virology , Chickens , Cattle Diseases/epidemiology , Cattle Diseases/transmission , Eggs , Enzyme-Linked Immunosorbent Assay , Genome, Viral , RNA, Viral , Seasons , Bluetongue virus/genetics , Bluetongue virus/immunology , Virus CultivationABSTRACT
Se aislaron blastos atípicos de la sangre periférica de un niño con síndrome de Wiskott-Aldrich y autotransplante de médula ósea. Las células fueron inmunotipificadas como monoblastos y crecieron en cultivo tisular en doble tiempo de 3 a 4 días. Las células aisladas originalmente y los cultivos iniciales, contenían antígenos tanto del virus herpes humano-6 (HHV-6) como del virus herpes humano-7 (HHV-7) y ácido desoxirribonucleico (ADN). Lo que disminuyó con la desdiferenciación celular en los cultivos subsecuentes. Los sobrenadantes de los cultivos celulares contenían cantidades moderadas de interleucina-6 (IL-6) y marcados niveles de factor estimulante de colonias-granulocito-monocítico (GM-CSF). Se discutió el caso desde el punto de vista de una posible co-patogénesis viral
Subject(s)
Humans , Male , Infant , Herpesviridae/immunology , Antigens, Viral , Cells, Cultured/immunology , Cells, Cultured/virology , Wiskott-Aldrich Syndrome/immunology , Wiskott-Aldrich Syndrome/blood , Wiskott-Aldrich Syndrome/virology , Transplantation, Autologous , Bone Marrow TransplantationABSTRACT
Infectivity titre of IBR virus in MDBK cells using monolayer, suspension and roller system revealed that the roller system produced the highest infectivity titre with an increase of log[10] 1.0 than their corresponding monolayer cell culture. On the other hand, the infectivity titres in cell suspension cultures were log[10] 0.6-log[10] 0.8 lower than their corresponding monolayer cell culture system. Influence of DEAE-dextran for the enhancement of IBR virus infectivity in different cell culture systems, 25 microg DEAE-dextran per ml is considered the safest concentration to the MDBK and VERO cell lines and giving these results: 1- Infectivity titre of IBR virus was markedly increased under the effect of both serial passages and DEAE-dextran in MDBK and VERO cells which showed an increase of log[10] 1.2 and log[10] 1.0, respectively more than their respective non treated passages. 2- In the roller system, DEAE-dextran enhanced the growth of virus by about 0.9, 0.7 log[10] more than growth of untreated virus in both MDBK and VERO cell lines, respectively. 3- The highest titre obtained when using DEAE-dextran MDBK roller system was log[10] 9.2 TCID[50]/ml